A non-binding contract is an agreement that has failed, either because one of the key elements of a valid contract is missing, or the content of the contract rendering it unenforceable by law. The non-binding dynamics of complexes with cell adhesion molecules depend on specific ligandens. Nuclear microscopy measurements showed that for P-Selectin-P-Selectin-Glycoprotein (sPSGL-1), the average lifespan of the t-link initially increases to low (10 pN (slip bonds) (catch bonds). On the other hand, for the complex with G1 anti-P-selectine, monotonous t monoclonal antibodies decrease with f. To quantitatively represent the energy landscape of these complexes, we use a model that takes into account the possibility of redistributing the population from one forceless state to another bound state destabilized by force. The excellent adequacy between theory and experiments allows us to extract the parameters of the energy landscape by adapting the calculated curves to the lifespan measurements for sPSGL-1 and G1. Surprisingly, the non-binding transition state for the P-selectin-G1 complex is close (0.32 nm) to the bound state, which means that the interaction is fragile, i.e. after deformation, complex fractures. In contrast, the non-binding transition state of the P-selectin-sPSGL-1 complex is far from the bound state (about 1.5 nm), indicating a compliant structure. Constant energy parameters are used to calculate the distribution of exit times and unrelated forces based on chargers, RF.
For a given RF, sPSGL-1 does not apply to a wider range of f, the most likely f being smaller than G1. The theory of cell adhesion complexes can be used to predict the results of non-association of other protein complexes. The distribution of non-binding times, p t (t), calculated for sPSGL-1… The moral of the story is that one party cannot automatically set aside a binding financial agreement simply because the other party violated it first. As in this case, the BFA may still be binding and the victim may have to seek alternative recourse in the event of a breach of a contractual clause.